Protein Info for b3746 in Escherichia coli BW25113

Name: yieN
Annotation: putative 2-component regulator (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 transmembrane" amino acids 28 to 49 (22 residues), see Phobius details PF20030: bpMoxR" amino acids 10 to 208 (199 residues), 189.6 bits, see alignment E=1.1e-59 PF07728: AAA_5" amino acids 42 to 171 (130 residues), 97.4 bits, see alignment E=2.3e-31 PF17868: AAA_lid_8" amino acids 227 to 298 (72 residues), 73.5 bits, see alignment E=3.1e-24 PF20265: LARA_dom" amino acids 335 to 436 (102 residues), 134.4 bits, see alignment E=5.1e-43 PF12592: ATPase_RavA_C" amino acids 444 to 497 (54 residues), 82.7 bits, see alignment 4.3e-27

Best Hits

Swiss-Prot: 100% identical to RAVA_ECOBW: ATPase RavA (ravA) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K03924, MoxR-like ATPase [EC: 3.6.3.-] (inferred from 100% identity to eco:b3746)

MetaCyc: 100% identical to regulatory ATPase RavA (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Putative regulator protein"

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P31473 at UniProt or InterPro

Protein Sequence (498 amino acids)

>b3746 putative 2-component regulator (VIMSS) (Escherichia coli BW25113)
MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFLLGPPGIAKSLIARRLK
FAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTSGYLPEAEIVFLDEIWKAG
PAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLIRLWLDKV
QDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFELIFMLRQQLD
KLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQSLNLIQQQIDV
LMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTVIRLGGIFSRRQQYQLPVNVTAS
TLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNGIGFAQKLNLEVDSAQHL
VVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPGDWLGR
IEASLQDVGAQIRQAQQC