Protein Info for b3722 in Escherichia coli BW25113

Name: bglF
Annotation: fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01995: PTS system, beta-glucoside-specific IIABC component
  • amino acids 3 to 623 (621 residues), 704.3 bits, E=2.8e-215
• PF00367: PTS_EIIB
  • amino acids 6 to 38 (33 residues), 56.5 bits, see alignment (E = 2.3e-19)
• TIGR00826: PTS system, glucose-like IIB component
  • amino acids 26 to 106 (81 residues), 54.6 bits, E=2.2e-18
• PF02378: PTS_EIIC
  • amino acids 106 to 389 (284 residues), 116.4 bits, E=2.3e-37
• TIGR00852: PTS system, maltose and glucose-specific subfamily, IIC component
  • amino acids 148 to 433 (286 residues), 295.8 bits, E=6.8e-92
• PF00358: PTS_EIIA_1
  • amino acids 479 to 602 (124 residues), 159 bits, E=6.7e-51
• TIGR00830: PTS system, glucose subfamily, IIA component
  • amino acids 479 to 599 (121 residues), 148.9 bits, E=1.3e-47

Best Hits

Swiss-Prot: 100% identical to PTV3B_ECOLI: PTS system beta-glucoside-specific EIIBCA component (bglF) from Escherichia coli (strain K12)

KEGG orthology group: K02755, PTS system, beta-glucosides-specific IIA component [EC: 2.7.1.69] K02756, PTS system, beta-glucosides-specific IIB component [EC: 2.7.1.69] K02757, PTS system, beta-glucosides-specific IIC component (inferred from 100% identity to eco:b3722)

MetaCyc: 100% identical to beta-glucoside specific PTS enzyme II / BglG kinase / BglG phosphatase (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-153A [EC: 2.7.1.199]; 2.7.1.199 [EC: 2.7.1.199]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
• glycolysis I (from glucose 6-phosphate) (13/13 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.199 or 2.7.1.69

Sequence Analysis Tools

See P08722 at UniProt or InterPro

Protein Sequence (625 amino acids)

>b3722 fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component (NCBI) (Escherichia coli BW25113)
MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQ
VVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVYVISGIFTPLIGLMAATGI
LKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGYTAGKRFGGNPFTAMVIGGA
LVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPS
AIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIF
VMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAA
LTSLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIFTFMQTIP
STGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGIC
SPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDD
GVEILIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNS
DDFTDVLPHGTAQISAGEPLLSIIR