Protein Info for b3600 in Escherichia coli BW25113

Name: mtlD
Annotation: mannitol-1-phosphate 5-dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01232: Mannitol_dh
  • amino acids 1 to 123 (123 residues), 103.8 bits, E=1e-33
• PF08125: Mannitol_dh_C
  • amino acids 148 to 373 (226 residues), 241.9 bits, E=7.5e-76

Best Hits

Swiss-Prot: 100% identical to MTLD_ECOLI: Mannitol-1-phosphate 5-dehydrogenase (mtlD) from Escherichia coli (strain K12)

KEGG orthology group: K00009, mannitol-1-phosphate 5-dehydrogenase [EC: 1.1.1.17] (inferred from 100% identity to eco:b3600)

MetaCyc: 100% identical to mannitol-1-phosphate 5-dehydrogenase (Escherichia coli K-12 substr. MG1655)
Mannitol-1-phosphate 5-dehydrogenase. [EC: 1.1.1.17]

Predicted SEED Role

"Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17)" in subsystem Mannitol Utilization (EC 1.1.1.17)

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
• superpathway of hexitol degradation (bacteria) (18/18 steps found)
• mannitol cycle (4/5 steps found)
• mannitol degradation I (1/1 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.17

Sequence Analysis Tools

See P09424 at UniProt or InterPro

Protein Sequence (382 amino acids)

>b3600 mannitol-1-phosphate 5-dehydrogenase (NCBI) (Escherichia coli BW25113)
MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARHSYQVHVVGETEQVDTV
SGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKGQVKRKEQGNESPLNIIAC
ENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRIVPPSASATNDPLEVTVETFS
EWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNTGHAITAYLGKLAGHQTIRDAIL
DEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQKILGRFENPYLKDDVERVGRQPLRK
LSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHFRSEDDPQAQELAALIADKGPQAALAQ
ISGLDANSEVVSEAVTAYKAMQ