Protein Info for b3546 in Escherichia coli BW25113

Name: yhjW
Annotation: orf, hypothetical protein (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 563 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 43 to 66 (24 residues), see Phobius details amino acids 77 to 104 (28 residues), see Phobius details amino acids 119 to 139 (21 residues), see Phobius details amino acids 160 to 178 (19 residues), see Phobius details PF08019: EptA_B_N" amino acids 59 to 170 (112 residues), 55.9 bits, see alignment E=4.7e-19 PF00884: Sulfatase" amino acids 249 to 538 (290 residues), 219.1 bits, see alignment E=8.7e-69

Best Hits

Swiss-Prot: 100% identical to EPTB_ECOLI: Kdo(2)-lipid A phosphoethanolamine 7''-transferase (eptB) from Escherichia coli (strain K12)

KEGG orthology group: K12975, phosphoethanolamine transferase (inferred from 100% identity to eco:b3546)

MetaCyc: 100% identical to phosphoethanolamine transferase EptB (Escherichia coli K-12 substr. MG1655)
RXN-16286 [EC: 2.7.8.42]; 2.7.8.42 [EC: 2.7.8.42]; 2.7.8.42 [EC: 2.7.8.42]

Predicted SEED Role

"Phosphoethanolamine transferase specific for the outer Kdo residue of lipopolysaccharide" in subsystem Lipid A modifications

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37661 at UniProt or InterPro

Protein Sequence (563 amino acids)

>b3546 orf, hypothetical protein (VIMSS) (Escherichia coli BW25113)
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTF
FLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVG
LNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRSLAVVVLAGIMVWAPIRLLDI
QQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRC
MFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGA
EPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSHFNYTQRYPRSFAQWKPECIG
VDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTS
PDGGINENNNWCHIPQAKEAAAN