Protein Info for b3416 in Escherichia coli BW25113

Name: malQ
Annotation: 4-alpha-glucanotransferase (amylomaltase) (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 694 PF21226: MalQ_N" amino acids 53 to 128 (76 residues), 59.8 bits, see alignment E=2.4e-20 TIGR00217: 4-alpha-glucanotransferase" amino acids 100 to 685 (586 residues), 1014 bits, see alignment E=0 PF02446: Glyco_hydro_77" amino acids 148 to 664 (517 residues), 443 bits, see alignment E=1.5e-136

Best Hits

Swiss-Prot: 100% identical to MALQ_ECOLI: 4-alpha-glucanotransferase (malQ) from Escherichia coli (strain K12)

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 100% identity to eco:b3416)

MetaCyc: 100% identical to 4-alpha-glucanotransferase (Escherichia coli K-12 substr. MG1655)
4-alpha-glucanotransferase. [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; MALTODEG-RXN [EC: 2.4.1.25]; RXN0-7347 [EC: 2.4.1.25]

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P15977 at UniProt or InterPro

Protein Sequence (694 amino acids)

>b3416 4-alpha-glucanotransferase (amylomaltase) (NCBI) (Escherichia coli BW25113)
MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQRTATKVAVTPVPNVMVY
TSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPTKLPEGYHTLTLTQDDQRA
HCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWGIGDFGDLKAMLVDVAKRGGS
FIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNAVEDFHLSEEAQAWWQLPTTQQT
LQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDEQMAAFRQFVAEQGDSLFWQAAFDA
LHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFCEEHRDDVDFYLWLQWLAYSQFAACWE
ISQGYEMPIGLYRDLAVGVAEGGAETWCDRELYCLKASVGAPPDILGPLGQNWGLPPMDP
HIITARAYEPFIELLRANMQNCGALRIDHVMSMLRLWWIPYGETADQGAYVHYPVDDLLS
ILALESKRHRCMVIGEDLGTVPVEIVGKLRSSGVYSYKVLYFENDHEKTFRAPKAYPEQS
MAVAATHDLPTLRGYWECGDLTLGKTLGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCL
PKRAGHKASLMSMTPTLNRGLQRYIADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWR
RKLSATLESMFADDGVNKLLKDLDRRRRAAAKKK