Protein Info for b3375 in Escherichia coli BW25113

Name: yhfR
Annotation: putative transcriptional regulator (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 243 PF00392: GntR" amino acids 12 to 75 (64 residues), 82.2 bits, see alignment E=2.4e-27 PF08220: HTH_DeoR" amino acids 39 to 75 (37 residues), 21.2 bits, see alignment 3e-08 PF07702: UTRA" amino acids 96 to 234 (139 residues), 113.6 bits, see alignment E=9.7e-37

Best Hits

Swiss-Prot: 100% identical to FRLR_ECOLI: Probable fructoselysine utilization operon transcriptional repressor (frlR) from Escherichia coli (strain K12)

KEGG orthology group: K10711, GntR family transcriptional regulator, frlABCD operon transcriptional regulator (inferred from 100% identity to eco:b3375)

Predicted SEED Role

"Transcriptional regulator of fructoselysine utilization operon FrlR"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P45544 at UniProt or InterPro

Protein Sequence (243 amino acids)

>b3375 putative transcriptional regulator (VIMSS) (Escherichia coli BW25113)
MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQYNVSRITIRKAISDLV
ADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATKEKVIEQERVSAAPFCEKL
NIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEIYVEGSSTYQLFQERFDTRVV
SDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQNDKPVHVSELFCRANRITLTIDN
KRH