Protein Info for b3169 in Escherichia coli BW25113

Name: nusA
Annotation: transcription elongation factor NusA (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF08529: NusA_N" amino acids 4 to 123 (120 residues), 133.2 bits, see alignment E=1.1e-42 TIGR01953: transcription termination factor NusA" amino acids 4 to 342 (339 residues), 433 bits, see alignment E=7.4e-134 PF00575: S1" amino acids 134 to 195 (62 residues), 22.5 bits, see alignment E=2.4e-08 PF13184: KH_5" amino acids 230 to 297 (68 residues), 99.3 bits, see alignment E=2.2e-32 TIGR01954: transcription termination factor NusA, C-terminal duplication" amino acids 365 to 414 (50 residues), 72.9 bits, see alignment 1.8e-24 amino acids 440 to 489 (50 residues), 64.9 bits, see alignment 5.3e-22 PF14520: HHH_5" amino acids 432 to 486 (55 residues), 43.3 bits, see alignment 8.5e-15

Best Hits

Swiss-Prot: 100% identical to NUSA_ECOL6: Transcription termination/antitermination protein NusA (nusA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02600, N utilization substance protein A (inferred from 100% identity to eco:b3169)

Predicted SEED Role

"Transcription termination protein NusA" in subsystem NusA-TFII Cluster or Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AFF6 at UniProt or InterPro

Protein Sequence (495 amino acids)

>b3169 transcription elongation factor NusA (NCBI) (Escherichia coli BW25113)
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFR
RWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVRE
AERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVR
GVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDE
DKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFT
KYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQA
QEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGA
LIMAARNICWFGDEA