Protein Info for b3132 in Escherichia coli BW25113

Name: kbaZ
Annotation: tagatose 6-phosphate aldolase 1, kbaZ subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08013: GatZ_KbaZ-like
  • amino acids 4 to 423 (420 residues), 668.9 bits, E=1.3e-205
• TIGR02810: D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit
  • amino acids 6 to 424 (419 residues), 719.1 bits, E=8.6e-221

Best Hits

Swiss-Prot: 100% identical to KBAZ_ECODH: D-tagatose-1,6-bisphosphate aldolase subunit KbaZ (kbaZ) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K00917, tagatose 6-phosphate kinase [EC: 2.7.1.144] (inferred from 100% identity to eco:b3132)

MetaCyc: 100% identical to putative tagatose-1,6-bisphosphate aldolase 1 chaperone (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Tagatose-6-phosphate kinase AgaZ (EC 2.7.1.144)" in subsystem N-Acetyl-Galactosamine and Galactosamine Utilization (EC 2.7.1.144)

MetaCyc Pathways

• superpathway of hexitol degradation (bacteria) (18/18 steps found)
• galactitol degradation (5/5 steps found)
• D-galactosamine and N-acetyl-D-galactosamine degradation (4/4 steps found)
• N-acetyl-D-galactosamine degradation (4/5 steps found)
• lactose degradation I (2/5 steps found)

KEGG Metabolic Maps

• Galactose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.144

Use Curated BLAST to search for 2.7.1.144

Sequence Analysis Tools

See P0C8K0 at UniProt or InterPro

Protein Sequence (426 amino acids)

>b3132 tagatose 6-phosphate aldolase 1, kbaZ subunit (NCBI) (Escherichia coli BW25113)
MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPLLIEATSNQVDQFGGYT
GMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPAAQAMANADDLIKSYVAAG
FKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCLEHFGEADLEYVIGTEVPVPG
GAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWPRIIALVVQPGVEFDHTNVIDYQ
PAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLVIDHFAILKVGPALTFALREALFSL
AAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSHYHGDGNARRLARGYSYSDRVRYYWPD
SQIDDAFAHLVRNLADSPIPLPLISQYLPLQYVKVRSGELQPTPRELIINHIQDILAQYH
TACEGQ