Protein Info for b3118 in Escherichia coli BW25113

Name: tdcA
Annotation: DNA-binding transcriptional activator (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 PF00126: HTH_1" amino acids 10 to 68 (59 residues), 68.6 bits, see alignment E=3.6e-23 PF03466: LysR_substrate" amino acids 98 to 297 (200 residues), 115.7 bits, see alignment E=2e-37

Best Hits

Swiss-Prot: 100% identical to TDCA_ECO57: HTH-type transcriptional regulator TdcA (tdcA) from Escherichia coli O157:H7

KEGG orthology group: K07592, LysR family transcriptional regulator, tdc operon transcriptional activator (inferred from 100% identity to eco:b3118)

Predicted SEED Role

"Threonine catabolic operon transcriptional activator TdcA" in subsystem Threonine anaerobic catabolism gene cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0ACQ7 at UniProt or InterPro

Protein Sequence (312 amino acids)

>b3118 DNA-binding transcriptional activator (NCBI) (Escherichia coli BW25113)
MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTG
VTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFK
EVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKLQDLHVEPLFESEFVLVASKS
RTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNA
DFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSY
NGCRRRQLIEVG