Protein Info for b3035 in Escherichia coli BW25113

Name: tolC
Annotation: outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 21 to 443 (423 residues), 481.6 bits, see alignment E=1.1e-148 PF02321: OEP" amino acids 28 to 210 (183 residues), 147.7 bits, see alignment E=1.7e-47 amino acids 234 to 428 (195 residues), 166.8 bits, see alignment E=2.3e-53

Best Hits

Swiss-Prot: 100% identical to TOLC_ECOLI: Outer membrane protein TolC (tolC) from Escherichia coli (strain K12)

KEGG orthology group: K12340, outer membrane channel protein TolC (inferred from 100% identity to eco:b3035)

MetaCyc: 100% identical to outer membrane channel TolC (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Type I secretion outer membrane protein, TolC precursor" in subsystem Multidrug Resistance Efflux Pumps or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P02930 at UniProt or InterPro

Protein Sequence (493 amino acids)

>b3035 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) (Escherichia coli BW25113)
MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAADRDAAFEKINEARSPL
LPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSKWRALTLQEKAAGIQDVTY
QTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLDQTTQRFNVGLVAITDVQNAR
AQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAALNVENFKTDKPQPVNALLKEAEK
RNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASTGISDTSYSGSKTRGAAGTQYDDSN
MGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGASEQLESAHRSVVQTVRSSFNNINASI
SSINAYKQAVVSAQSSLDAMEAGYSVGTRTIVDVLDATTTLYNAKQELANARYNYLINQL
NIKSALGTLNEQDLLALNNALSKPVSTNPENVAPQTPEQNAIADGYAPDSPAPVVQQTSA
RTTTSNGHNPFRN