Protein Info for b2996 in Escherichia coli BW25113

Name: hybA
Annotation: hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 293 to 311 (19 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 2 to 26 (25 residues), 18.4 bits, see alignment (E = 1.1e-07) PF13247: Fer4_11" amino acids 106 to 207 (102 residues), 80.1 bits, see alignment E=4.1e-26 PF12838: Fer4_7" amino acids 111 to 159 (49 residues), 31.4 bits, see alignment 7.7e-11 PF12797: Fer4_2" amino acids 137 to 157 (21 residues), 26 bits, see alignment (E = 2.1e-09) PF12837: Fer4_6" amino acids 137 to 160 (24 residues), 28.8 bits, see alignment (E = 3.1e-10) PF00037: Fer4" amino acids 139 to 160 (22 residues), 25.4 bits, see alignment (E = 3.2e-09)

Best Hits

Swiss-Prot: 100% identical to HYBA_SHIFL: Hydrogenase-2 operon protein HybA (hybA) from Shigella flexneri

KEGG orthology group: None (inferred from 100% identity to eco:b2996)

MetaCyc: 100% identical to hydrogenase 2 iron-sulfur protein (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Fe-S-cluster-containing hydrogenase components 1" in subsystem Anaerobic respiratory reductases or Hydrogenases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AAJ8 at UniProt or InterPro

Protein Sequence (328 amino acids)

>b2996 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) (Escherichia coli BW25113)
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
LAVLAGLTVLVRRNTKNDHHDGGDDHES