Protein Info for b2988 in Escherichia coli BW25113

Name: gss
Annotation: fused glutathionylspermidine amidase/glutathionylspermidine synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 619 PF05257: CHAP" amino acids 52 to 149 (98 residues), 40.5 bits, see alignment E=3.1e-14 PF03738: GSP_synth" amino acids 251 to 615 (365 residues), 219.2 bits, see alignment E=1e-68

Best Hits

Swiss-Prot: 100% identical to GSP_SHIFL: Bifunctional glutathionylspermidine synthetase/amidase (gss) from Shigella flexneri

KEGG orthology group: K01460, glutathionylspermidine amidase/synthetase [EC: 3.5.1.78 6.3.1.8] (inferred from 100% identity to eco:b2988)

MetaCyc: 100% identical to fused glutathionylspermidine amidase / glutathionylspermidine synthetase (Escherichia coli K-12 substr. MG1655)
Glutathionylspermidine synthase. [EC: 6.3.1.8, 6.3.1.9]; Glutathionylspermidine amidase. [EC: 6.3.1.8, 6.3.1.9, 3.5.1.78]

Predicted SEED Role

"Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78)" in subsystem Glutathionylspermidine and Trypanothione (EC 3.5.1.78, EC 6.3.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.8

Use Curated BLAST to search for 3.5.1.78 or 6.3.1.8 or 6.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AES0 at UniProt or InterPro

Protein Sequence (619 amino acids)

>b2988 fused glutathionylspermidine amidase/glutathionylspermidine synthetase (NCBI) (Escherichia coli BW25113)
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCV
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTF
DDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFN
PAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELG
WDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCG
SNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
ESLVIKKESDIEPLIVVKK