Protein Info for b2965 in Escherichia coli BW25113

Name: speC
Annotation: ornithine decarboxylase isozyme (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 711 PF03709: OKR_DC_1_N" amino acids 4 to 95 (92 residues), 56.9 bits, see alignment E=3.5e-19 PF01276: OKR_DC_1" amino acids 101 to 543 (443 residues), 671.5 bits, see alignment E=6.6e-206 PF03711: OKR_DC_1_C" amino acids 568 to 697 (130 residues), 155.4 bits, see alignment E=1.5e-49

Best Hits

Swiss-Prot: 100% identical to DCOR_ECOLI: Constitutive ornithine decarboxylase (speC) from Escherichia coli (strain K12)

KEGG orthology group: K01581, ornithine decarboxylase [EC: 4.1.1.17] (inferred from 100% identity to ecx:EcHS_A3126)

MetaCyc: 100% identical to constitutive ornithine decarboxylase (Escherichia coli K-12 substr. MG1655)
Ornithine decarboxylase. [EC: 4.1.1.17]

Predicted SEED Role

"Ornithine decarboxylase (EC 4.1.1.17)" in subsystem Arginine and Ornithine Degradation or Polyamine Metabolism (EC 4.1.1.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.17

Use Curated BLAST to search for 4.1.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P21169 at UniProt or InterPro

Protein Sequence (711 amino acids)

>b2965 ornithine decarboxylase isozyme (VIMSS) (Escherichia coli BW25113)
MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSRSGILALLKRTGFHLPV
FLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYDTLTQYVEMGNSTFAC
PGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLIHEGSAKDAQKFAAKVFH
ADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHHGALIQAGATPVYLEASRNP
FGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYRLAIIQLGTYDGTVYNARQVIDTVG
HLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDPGIFVTQSVHKQQAGFSQTSQIHK
KDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFAALDVNAKIHEGESGRRLWAECVEIG
IEARKAILARCKLFRPFIPPVVDGKLWQDYPTSVLASDRRFFSFEPGAKWHGFEGYAADQ
YFVDPCKLLLTTPGIDAETGEYSDFGVPATILAHYLRENGIVPEKCDLNSILFLLTPAES
HEKLAQLVAMLAQFEQHIEDDSPLVEVLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDV
KDLQKAMFRQQSFPSVVMNPQDAHSAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVV
PGEVWGGAVQRYFLALEEGVNLLPGFSPELQGVYSETDADGVKRLYGYVLK