Protein Info for b2917 in Escherichia coli BW25113

Name: yliK
Annotation: methylmalonyl-CoA mutase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 27 to 547 (521 residues), 938 bits, E=1.1e-286
• PF01642: MM_CoA_mutase
  • amino acids 28 to 539 (512 residues), 779 bits, E=2e-238
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 583 to 713 (131 residues), 217.1 bits, E=6.6e-69
• PF02310: B12-binding
  • amino acids 586 to 701 (116 residues), 70.4 bits, E=1.2e-23

Best Hits

Swiss-Prot: 100% identical to SCPA_ECOLI: Methylmalonyl-CoA mutase (scpA) from Escherichia coli (strain K12)

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 100% identity to eco:b2917)

MetaCyc: 100% identical to methylmalonyl-CoA mutase (Escherichia coli K-12 substr. MG1655)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• conversion of succinate to propanoate (3/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• pyruvate fermentation to propanoate I (5/7 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• L-glutamate degradation VIII (to propanoate) (5/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• methylaspartate cycle (9/19 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See P27253 at UniProt or InterPro

Protein Sequence (714 amino acids)

>b2917 methylmalonyl-CoA mutase (NCBI) (Escherichia coli BW25113)
MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADLDNLEVTGTLPGLPPYV
RGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDNPR
VAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEEQGVTPDK
LTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISGYHMGEAGAN
CVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAMLRAARYLWSEA
VSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNVIRTTIEALAATLGGTQSLHTNAFDEA
LGLPTDFSARIARNTQIIIQEESELCRTVDPLAGSYYIESLTDQIVKQARAIIQQIDEAG
GMAKAIEAGLPKRMIEEASAREQSLIDQGKRVIVGVNKYKLDHEDETDVLEIDNVMVRNE
QIASLERIRATRDDAAVTAALNALTHAAQHNENLLAAAVNAARVRATLGEISDALEVAFD
RYLVPSQCVTGVIAQSYHQSEKSASEFDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRG
AKVIASAYSDLGFDVDLSPMFSTPEEIARLAVENDVHVVGASSLAAGHKTLIPELVEALK
KWGREDICVVAGGVIPPQDYAFLQERGVAAIYGPGTPMLDSVRDVLNLISQHHD