Protein Info for b2913 in Escherichia coli BW25113

Name: serA
Annotation: D-3-phosphoglycerate dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 14 to 326 (313 residues), 132.9 bits, E=1.2e-42
• PF02826: 2-Hacid_dh_C
  • amino acids 119 to 294 (176 residues), 166.7 bits, E=6.9e-53
• PF01842: ACT
  • amino acids 344 to 401 (58 residues), 29.5 bits, E=1e-10

Best Hits

Swiss-Prot: 100% identical to SERA_ECO57: D-3-phosphoglycerate dehydrogenase (serA) from Escherichia coli O157:H7

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 100% identity to eco:b2913)

MetaCyc: 100% identical to phosphoglycerate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Phosphoglycerate dehydrogenase. [EC: 1.1.1.95]; KETOGLUTREDUCT-RXN [EC: 1.1.1.95, 1.1.1.399]; 1.1.1.399 [EC: 1.1.1.95, 1.1.1.399]

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.399 or 1.1.1.95

Sequence Analysis Tools

See P0A9T0 at UniProt or InterPro

Protein Sequence (410 amino acids)

>b2913 D-3-phosphoglycerate dehydrogenase (NCBI) (Escherichia coli BW25113)
MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDDEQLKESIRDAHFIGLR
SRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVFNAPFSNTRSVAELVIGEL
LLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYGHIGTQLGILAESLGMYVYFY
DIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTKNMMGAKEISLMKPGSLLINASR
GTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDPFTSPLCEFDNVLLTPHIGGSTQEA
QENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPLHGGRRLMHIHENRPGVLTALNKIFAE
QGVNIAAQYLQTSAQMGYVVIDIEADEDVAEKALQAMKAIPGTIRARLLY