Protein Info for b2891 in Escherichia coli BW25113

Name: prfB
Annotation: peptide chain release factor RF-2 (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 TIGR00020: peptide chain release factor 2" amino acids 3 to 363 (361 residues), 601.1 bits, see alignment E=3.5e-185 PF03462: PCRF" amino acids 31 to 219 (189 residues), 208.1 bits, see alignment E=1.2e-65 PF00472: RF-1" amino acids 228 to 337 (110 residues), 142.8 bits, see alignment E=4.4e-46

Best Hits

Swiss-Prot: 100% identical to RF2_ECODH: Peptide chain release factor 2 (prfB) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K02836, peptide chain release factor 2 (inferred from 100% identity to eco:b2891)

Predicted SEED Role

"Peptide chain release factor 2; programmed frameshift-containing"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P07012 at UniProt or InterPro

Protein Sequence (365 amino acids)

>b2891 peptide chain release factor RF-2 (NCBI) (Escherichia coli BW25113)
MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERAQALGKER
SSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAELDALEEKLAQLEFRRMFS
GEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVT
IKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLR
IDVYRTSGAGGQHVNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEM
QKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEAS
LKAGL