Protein Info for b2806 in Escherichia coli BW25113

Name: ygdE
Annotation: predicted methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF18125: RlmM_FDX
  • amino acids 1 to 71 (71 residues), 101.2 bits, E=5.5e-33
• PF21239: RLMM_N
  • amino acids 84 to 164 (81 residues), 127.9 bits, E=1.9e-41
• PF01728: FtsJ
  • amino acids 186 to 279 (94 residues), 40.9 bits, E=3.2e-14

Best Hits

Swiss-Prot: 100% identical to RLMM_ECO5E: Ribosomal RNA large subunit methyltransferase M (rlmM) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K06968, ribosomal RNA large subunit methyltransferase M [EC: 2.1.1.-] (inferred from 100% identity to eco:b2806)

MetaCyc: 100% identical to 23S rRNA 2'-O-ribose C2498 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11589 [EC: 2.1.1.186]

Predicted SEED Role

"LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM"

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.186

Sequence Analysis Tools

See P0ADR6 at UniProt or InterPro

Protein Sequence (366 amino acids)

>b2806 predicted methyltransferase (NCBI) (Escherichia coli BW25113)
MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIYECYQPDDGDKLIRELP
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFC
RKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYTGYSYSNNNSPFYMGIPRLKF
PADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVCDMVEKPAKVAALMAQWLVNGWCRE
TIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVG
GRRDER