Protein Info for b2798 in Escherichia coli BW25113

Name: ygdG
Annotation: Ssb-binding protein, misidentified as ExoIX (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 251 PF02739: 5_3_exonuc_N" amino acids 5 to 142 (138 residues), 99.7 bits, see alignment E=1.5e-32 PF01367: 5_3_exonuc" amino acids 160 to 249 (90 residues), 94.8 bits, see alignment E=4e-31

Best Hits

Swiss-Prot: 100% identical to XNI_ECOBW: Flap endonuclease Xni (xni) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K01146, protein Xni (inferred from 100% identity to eco:b2798)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P38506 at UniProt or InterPro

Protein Sequence (251 amino acids)

>b2798 Ssb-binding protein, misidentified as ExoIX (RefSeq) (Escherichia coli BW25113)
MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRH
QRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNEADDLAATLAVKVTQAGHQ
ATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVP
GVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHI
DGNLQQLRLVR