Protein Info for b2704 in Escherichia coli BW25113

Name: srlB
Annotation: glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 123 TIGR00849: PTS system, glucitol/sorbitol-specific IIA component" amino acids 1 to 123 (123 residues), 202.2 bits, see alignment E=9.1e-65 PF03829: PTSIIA_gutA" amino acids 4 to 115 (112 residues), 130 bits, see alignment E=2e-42

Best Hits

Swiss-Prot: 100% identical to PTHA_ECOLI: PTS system glucitol/sorbitol-specific EIIA component (srlB) from Escherichia coli (strain K12)

KEGG orthology group: K02781, PTS system, glucitol/sorbitol-specific IIA component [EC: 2.7.1.69] (inferred from 100% identity to eco:b2704)

MetaCyc: 100% identical to sorbitol-specific PTS enzyme IIA component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-169 [EC: 2.7.1.198]; TRANS-RXN-156 [EC: 2.7.1.198, 2.7.1.197]

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.197, 2.7.1.69

Use Curated BLAST to search for 2.7.1.197 or 2.7.1.198 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P05706 at UniProt or InterPro

Protein Sequence (123 amino acids)

>b2704 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) (Escherichia coli BW25113)
MTVIYQTTITRIGASAIDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFSL
GQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFES
VKE