Protein Info for b2660 in Escherichia coli BW25113

Name: ygaF
Annotation: orf, hypothetical protein (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to LHGD_ECOLI: L-2-hydroxyglutarate dehydrogenase (lhgD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b2660)

MetaCyc: 100% identical to L-2-hydroxyglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-7319 [EC: 1.1.5.13]

Predicted SEED Role

"L-2-hydroxyglutarate oxidase (EC 1.1.3.15)" (EC 1.1.3.15)

MetaCyc Pathways

L-lysine degradation I (7/7 steps found)
photorespiration I (5/9 steps found)
superpathway of L-lysine degradation (14/43 steps found)

KEGG Metabolic Maps

Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.3.15

Use Curated BLAST to search for 1.1.3.15 or 1.1.5.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37339 at UniProt or InterPro

Protein Sequence (422 amino acids)

>b2660 orf, hypothetical protein (VIMSS) (Escherichia coli BW25113)
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGS
LKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLN
ADELREREPNITGLGGIFVPSSGIVSYRDVTAAMAKIFQSRGGEIIYNAEVSGLNEHKNG
VVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDF
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
NQ