Protein Info for b2575 in Escherichia coli BW25113

Name: yfiC
Annotation: putative enzyme (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 PF01596: Methyltransf_3" amino acids 21 to 119 (99 residues), 31.3 bits, see alignment E=6.4e-11 PF03602: Cons_hypoth95" amino acids 39 to 130 (92 residues), 32.1 bits, see alignment E=4.6e-11 PF01135: PCMT" amino acids 44 to 108 (65 residues), 23 bits, see alignment E=3e-08 PF05175: MTS" amino acids 46 to 129 (84 residues), 48.9 bits, see alignment E=3e-16 PF06325: PrmA" amino acids 46 to 122 (77 residues), 25.1 bits, see alignment E=6e-09 PF13847: Methyltransf_31" amino acids 47 to 128 (82 residues), 29.4 bits, see alignment E=3.1e-10 PF13649: Methyltransf_25" amino acids 49 to 122 (74 residues), 35 bits, see alignment E=9.4e-12 PF08241: Methyltransf_11" amino acids 49 to 122 (74 residues), 22.3 bits, see alignment E=8.3e-08 PF05185: PRMT5" amino acids 51 to 121 (71 residues), 23.5 bits, see alignment E=2.2e-08 PF01170: UPF0020" amino acids 65 to 142 (78 residues), 26.5 bits, see alignment E=2.4e-09

Best Hits

Swiss-Prot: 100% identical to TRMN6_ECOHS: tRNA1(Val) (adenine(37)-N6)-methyltransferase (yfiC) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: None (inferred from 100% identity to eco:b2575)

MetaCyc: 100% identical to tRNA m6A37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-12480 [EC: 2.1.1.223]

Predicted SEED Role

"tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223)" (EC 2.1.1.223)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.223

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P31825 at UniProt or InterPro

Protein Sequence (245 amino acids)

>b2575 putative enzyme (VIMSS) (Escherichia coli BW25113)
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
MLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
SNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM