Protein Info for b2542 in Escherichia coli BW25113

Name: hcaD
Annotation: phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 5 to 299 (295 residues), 198.2 bits, see alignment E=1.1e-61 PF00070: Pyr_redox" amino acids 146 to 222 (77 residues), 60.4 bits, see alignment E=1e-19 PF14759: Reductase_C" amino acids 318 to 400 (83 residues), 76.1 bits, see alignment E=1.3e-24

Best Hits

Swiss-Prot: 100% identical to HCAD_ECODH: 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component (hcaD) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K00529, ferredoxin--NAD+ reductase [EC: 1.18.1.3] (inferred from 100% identity to eco:b2542)

MetaCyc: 100% identical to putative 3-phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit (Escherichia coli K-12 substr. MG1655)
3-phenylpropanoate dioxygenase. [EC: 1.14.12.19]; 1.14.12.19 [EC: 1.14.12.19]

Predicted SEED Role

"3-phenylpropionate dioxygenase ferredoxin--NAD(+) reductase component (EC 1.18.1.3)" in subsystem Cinnamic Acid Degradation or Phenylpropionate Degradation (EC 1.18.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.19

Use Curated BLAST to search for 1.14.12.19 or 1.18.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77650 at UniProt or InterPro

Protein Sequence (400 amino acids)

>b2542 phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI) (Escherichia coli BW25113)
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQ
QVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA
LGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELAASATQRRCKVTVIELAATVM
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ
IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
IGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL