Protein Info for b2538 in Escherichia coli BW25113

Name: hcaE
Annotation: 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 43 to 125 (83 residues), 73.3 bits, E=1.2e-24
• PF00848: Ring_hydroxyl_A
  • amino acids 165 to 433 (269 residues), 118.2 bits, E=4.8e-38

Best Hits

Swiss-Prot: 100% identical to HCAE_ECO57: 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha (hcaE) from Escherichia coli O157:H7

KEGG orthology group: K05708, large terminal subunit of phenylpropionate dioxygenase [EC: 1.14.12.19] (inferred from 100% identity to eco:b2538)

MetaCyc: 100% identical to putative 3-phenylpropionate/cinnamate dioxygenase subunit alpha (Escherichia coli K-12 substr. MG1655)
3-phenylpropanoate dioxygenase. [EC: 1.14.12.19]; 1.14.12.19 [EC: 1.14.12.19]

Predicted SEED Role

"3-phenylpropionate dioxygenase, alpha subunit (EC 1.14.12.19)" in subsystem Cinnamic Acid Degradation or Flavohaemoglobin or Phenylpropionate Degradation (EC 1.14.12.19)

MetaCyc Pathways

• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (8/8 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (5/5 steps found)
• cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (5/5 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.19

Use Curated BLAST to search for 1.14.12.19

Sequence Analysis Tools

See P0ABR5 at UniProt or InterPro

Protein Sequence (453 amino acids)

>b2538 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI) (Escherichia coli BW25113)
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFN
TYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELID
VPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAPGLRDYLGDIAWYLDGMLDR
REGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRA
LRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRA
FGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQEKRRDDGIPGITNYIFSETA
ARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK