Protein Info for b2501 in Escherichia coli BW25113

Name: ppk
Annotation: polyphosphate kinase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 688 PF13089: PP_kinase_N" amino acids 7 to 109 (103 residues), 104.1 bits, see alignment E=1.3e-33 TIGR03705: polyphosphate kinase 1" amino acids 7 to 679 (673 residues), 853.1 bits, see alignment E=7.8e-261 PF02503: PP_kinase" amino acids 119 to 303 (185 residues), 156.5 bits, see alignment E=1.5e-49 PF17941: PP_kinase_C_1" amino acids 333 to 495 (163 residues), 246.9 bits, see alignment E=2e-77 PF13091: PLDc_2" amino acids 369 to 489 (121 residues), 36.8 bits, see alignment E=7.8e-13 PF13090: PP_kinase_C" amino acids 503 to 674 (172 residues), 218 bits, see alignment E=1.6e-68

Best Hits

Swiss-Prot: 100% identical to PPK1_ECOLI: Polyphosphate kinase (ppk) from Escherichia coli (strain K12)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to ecv:APECO1_4068)

MetaCyc: 100% identical to polyphosphate kinase (Escherichia coli K-12 substr. MG1655)
Polyphosphate kinase. [EC: 2.7.4.1]

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0A7B1 at UniProt or InterPro

Protein Sequence (688 amino acids)

>b2501 polyphosphate kinase (NCBI) (Escherichia coli BW25113)
MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFAELKRR
IIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEMARNQIFLINERQLSVNQQ
NWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVEIIRGDTIRYALLEIPSDKVP
RFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYDALNAYSMKMTRDAEYDLVHEME
ASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVLREKLTISRYDSIVPGGRYHNFKDF
INFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFDAIRERDVLLYYPYHTFEHVLELLRQA
SFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVELQARFDEEANIHWAKRLTE
AGVHVIFSAPGLKIHAKLFLISRKENGEVVRYAHIGTGNFNEKTARLYTDYSLLTADARI
TNEVRRVFNFIENPYRPVTFDYLMVSPQNSRRLLYEMVDREIANAQQGLPSGITLKLNNL
VDKGLVDRLYAASSSGVPVNLLVRGMCSLIPNLEGISDNIRAISIVDRYLEHDRVYIFEN
GGDKKVYLSSADWMTRNIDYRIEVATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSN
RYVPRGNRRKVRAQLAIYDYIKSLEQPE