Protein Info for b2459 in Escherichia coli BW25113
Name: eutT
Annotation: predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to EUTT_ECOL6: Ethanolamine utilization cobalamin adenosyltransferase (eutT) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K04032, ethanolamine utilization cobalamin adenosyltransferase [EC: 2.5.1.17] (inferred from 100% identity to eco:b2459)MetaCyc: 89% identical to corrinoid adenosyltransferase EutT monomer (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN-19336 [EC: 2.5.1.154]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
- adenosylcobalamin salvage from cobalamin (5/5 steps found)
- adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (7/9 steps found)
- adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (4/6 steps found)
- adenosylcobinamide-GDP salvage from cobinamide II (4/6 steps found)
- cobalamin salvage (eukaryotic) (4/8 steps found)
- adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (16/36 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.17
Use Curated BLAST to search for 2.5.1.154 or 2.5.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P65643 at UniProt or InterPro
Protein Sequence (267 amino acids)
>b2459 predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI) (Escherichia coli BW25113) MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLRIKFIDEQGRLFVDDEQ QQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKMVAKSDPRLGFRAVLDSTI ALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGCQAIVGLSDEDLHRLSHQPLR YLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVFITRSFEVLRPDILQALNRLSST VYVMMILSVTKQPLTVKQIQQRLGETQ