Protein Info for b2441 in Escherichia coli BW25113

Name: eutB
Annotation: ethanolamine ammonia-lyase, heavy chain (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 PF06751: EutB" amino acids 12 to 444 (433 residues), 682.8 bits, see alignment E=1e-209

Best Hits

Swiss-Prot: 100% identical to EUTB_ECO57: Ethanolamine ammonia-lyase heavy chain (eutB) from Escherichia coli O157:H7

KEGG orthology group: K03735, ethanolamine ammonia-lyase large subunit [EC: 4.3.1.7] (inferred from 100% identity to eco:b2441)

MetaCyc: 100% identical to ethanolamine ammonia-lyase subunit alpha (Escherichia coli K-12 substr. MG1655)
Ethanolamine ammonia-lyase. [EC: 4.3.1.7]

Predicted SEED Role

"Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7)" in subsystem Ethanolamine utilization (EC 4.3.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.7

Use Curated BLAST to search for 4.3.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AEJ6 at UniProt or InterPro

Protein Sequence (453 amino acids)

>b2441 ethanolamine ammonia-lyase, heavy chain (VIMSS) (Escherichia coli BW25113)
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
NNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
VVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEG
LSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANF
GADQVTMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
LRPSPEFERWLESMGIMANGRLTKRAGDPSLFF