Protein Info for b2370 in Escherichia coli BW25113

Name: evgS
Annotation: hybrid sensory histidine kinase in two-component regulatory system with EvgA (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1197 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 536 to 558 (23 residues), see Phobius details PF00497: SBP_bac_3" amino acids 72 to 276 (205 residues), 57.8 bits, see alignment E=3.1e-19 amino acids 306 to 520 (215 residues), 95.3 bits, see alignment E=1e-30 PF00512: HisKA" amino acids 711 to 777 (67 residues), 60.1 bits, see alignment 4.6e-20 PF02518: HATPase_c" amino acids 825 to 937 (113 residues), 95.3 bits, see alignment E=7.7e-31 PF00072: Response_reg" amino acids 961 to 1070 (110 residues), 103.9 bits, see alignment E=1.4e-33 PF01627: Hpt" amino acids 1104 to 1186 (83 residues), 26.1 bits, see alignment 2.1e-09

Best Hits

Swiss-Prot: 100% identical to EVGS_ECOLI: Sensor protein EvgS (evgS) from Escherichia coli (strain K12)

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 100% identity to eco:b2370)

MetaCyc: 100% identical to sensor histidine kinase EvgS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P30855 at UniProt or InterPro

Protein Sequence (1197 amino acids)

>b2370 hybrid sensory histidine kinase in two-component regulatory system with EvgA (NCBI) (Escherichia coli BW25113)
MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRLSNKELRWLASKKNLVI
AVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLREYADHQKAMDALAEGEVDI
VLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLTSPKPVNIARVANYPPDEVIH
QSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSSMISRYFTHSLNVVKYYNSPRQY
NFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQ
HPNLKVLENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLSP
GGWDIIPGAIYSEDRENNVLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHS
QLKEMYPEVEWIQVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVP
NASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVT
TLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVIS
HNSAFEHYFTADYYKNAMLPLENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIE
KRCINHWHTLCNLPASDNAVYICGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMS
HEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQ
LQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNA
LKFTTEGAVKITTSLGHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGS
GLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLS
ILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFEL
TRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIA
PQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIH
GAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND