Protein Info for b2293 in Escherichia coli BW25113

Name: yfbT
Annotation: putative phosphatase (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 PF00702: Hydrolase" amino acids 4 to 175 (172 residues), 103.1 bits, see alignment E=5.3e-33 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 6 to 181 (176 residues), 73.2 bits, see alignment E=2.6e-24 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 6 to 163 (158 residues), 23.7 bits, see alignment E=5.5e-09 PF12710: HAD" amino acids 7 to 127 (121 residues), 38.8 bits, see alignment E=2.9e-13 PF13419: HAD_2" amino acids 7 to 181 (175 residues), 95.3 bits, see alignment E=9.7e-31 PF13242: Hydrolase_like" amino acids 136 to 183 (48 residues), 30.7 bits, see alignment 4.7e-11

Best Hits

Swiss-Prot: 100% identical to HXPA_ECOLI: Hexitol phosphatase A (hxpA) from Escherichia coli (strain K12)

KEGG orthology group: K01112, [EC: 3.1.3.-] (inferred from 100% identity to eco:b2293)

MetaCyc: 100% identical to hexitol phosphatase A (Escherichia coli K-12 substr. MG1655)
Sorbitol-6-phosphatase. [EC: 3.1.3.50]; Sugar-phosphatase. [EC: 3.1.3.50, 3.1.3.23]; Mannitol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22]; Glycerol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22, 3.1.3.21]

Predicted SEED Role

"Putative phosphatase YfbT" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.3.23

Use Curated BLAST to search for 3.1.3.- or 3.1.3.21 or 3.1.3.22 or 3.1.3.23 or 3.1.3.50

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77625 at UniProt or InterPro

Protein Sequence (216 amino acids)

>b2293 putative phosphatase (VIMSS) (Escherichia coli BW25113)
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
EADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPWAIVTSGSMPVARARHKIA
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ