Protein Info for b2268 in Escherichia coli BW25113

Name: elaC
Annotation: orf, hypothetical protein (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 TIGR02649: ribonuclease BN" amino acids 2 to 305 (304 residues), 689.8 bits, see alignment E=3.4e-212 TIGR02651: ribonuclease Z" amino acids 2 to 304 (303 residues), 346.4 bits, see alignment E=1.2e-107 PF00753: Lactamase_B" amino acids 30 to 135 (106 residues), 30.7 bits, see alignment E=3.1e-11 PF12706: Lactamase_B_2" amino acids 36 to 154 (119 residues), 34.3 bits, see alignment E=1.9e-12

Best Hits

Swiss-Prot: 100% identical to RBN_ECOHS: Ribonuclease BN (rbn) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K00784, ribonuclease Z [EC: 3.1.26.11] (inferred from 100% identity to eco:b2268)

MetaCyc: 100% identical to ribonuclease BN (Escherichia coli K-12 substr. MG1655)
Ribonuclease Z. [EC: 3.1.26.11]; Exoribonuclease II. [EC: 3.1.26.11, 3.1.13.1]; 3.1.13.1 [EC: 3.1.26.11, 3.1.13.1]

Predicted SEED Role

"Ribonuclease Z (EC 3.1.26.11)" in subsystem tRNA processing (EC 3.1.26.11)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 3.1.13.1 or 3.1.26.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0A8V0 at UniProt or InterPro

Protein Sequence (305 amino acids)

>b2268 orf, hypothetical protein (VIMSS) (Escherichia coli BW25113)
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKI
FISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVE
IGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALNAQALKAAGVPPGPLFQELKA
GKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITME
AKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDF
TVFNV