Protein Info for b2251 in Escherichia coli BW25113

Name: yfaO
Annotation: predicted NUDIX hydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00293: NUDIX
  • amino acids 1 to 136 (136 residues), 81.8 bits, E=2.4e-27

Best Hits

Swiss-Prot: 100% identical to NUDI_ECOLC: Nucleoside triphosphatase NudI (nudI) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K12944, nucleoside triphosphatase [EC: 3.6.1.-] (inferred from 100% identity to eco:b2251)

MetaCyc: 100% identical to pyrimidine deoxynucleotide diphosphatase NudI (Escherichia coli K-12 substr. MG1655)
dCTP diphosphatase. [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9]; 3.6.1.9 [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9]; RXN-17104 [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9, 3.6.1.68]; dUTP diphosphatase. [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9, 3.6.1.68, 3.6.1.23]

Predicted SEED Role

"Pyrimidine deoxynucleoside triphosphate (dYTP) pyrophosphohydrolase YfoO" in subsystem Nudix proteins (nucleoside triphosphate hydrolases)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides dephosphorylation (3/3 steps found)
• geraniol biosynthesis (cytosol) (1/2 steps found)
• dZTP biosynthesis (3/5 steps found)
• juvenile hormone III biosynthesis I (1/6 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Riboflavin metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.23

Use Curated BLAST to search for 3.6.1.- or 3.6.1.12 or 3.6.1.23 or 3.6.1.65 or 3.6.1.68 or 3.6.1.9

Sequence Analysis Tools

See P52006 at UniProt or InterPro

Protein Sequence (141 amino acids)

>b2251 predicted NUDIX hydrolase (NCBI) (Escherichia coli BW25113)
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWAISGGGVEPGERIEEALRREIREELGE
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
DLVHYDLNVATRKTLRLKGLL