Protein Info for b2233 in Escherichia coli BW25113

Name: yfaL
Annotation: adhesin (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1250 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01376: chlamydial polymorphic outer membrane protein repeat" amino acids 124 to 146 (23 residues), 19.9 bits, see alignment (E = 7.4e-08) amino acids 228 to 249 (22 residues), 20 bits, see alignment (E = 6.6e-08) PF02415: Chlam_PMP" amino acids 127 to 145 (19 residues), 19 bits, see alignment (E = 2.5e-07) amino acids 186 to 208 (23 residues), 12.7 bits, see alignment (E = 2.7e-05) amino acids 228 to 245 (18 residues), 16.8 bits, see alignment (E = 1.3e-06) TIGR02601: autotransporter-associated beta strand repeat" amino acids 328 to 359 (32 residues), 31.1 bits, see alignment (E = 2.3e-11) PF12951: PATR" amino acids 328 to 357 (30 residues), 26.2 bits, see alignment (E = 1.4e-09) amino acids 559 to 580 (22 residues), 17 bits, see alignment (E = 1e-06) amino acids 663 to 690 (28 residues), 20.3 bits, see alignment (E = 9.4e-08) PF18883: AC_1" amino acids 794 to 904 (111 residues), 63.5 bits, see alignment E=3.8e-21 TIGR01414: outer membrane autotransporter barrel domain" amino acids 807 to 1250 (444 residues), 388.3 bits, see alignment E=7.9e-120 PF03797: Autotransporter" amino acids 989 to 1228 (240 residues), 120.3 bits, see alignment E=2.5e-38

Best Hits

Swiss-Prot: 98% identical to YFAL_ECOLI: Probable autotransporter YfaL (yfaL) from Escherichia coli (strain K12)

KEGG orthology group: K07279, hypothetical protein (inferred from 98% identity to eco:b2233)

Predicted SEED Role

"COG3468: Type V secretory pathway, adhesin AidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P45508 at UniProt or InterPro

Protein Sequence (1250 amino acids)

>b2233 adhesin (NCBI) (Escherichia coli BW25113)
MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQASRQSLSGITQDWSIA
DGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGGAIFAKEN
STLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAMFRNNIANDGKGGAIYTINN
DVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIVNNTAFTNNTAEGYG
GAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSSAAGGFMYLGLSEVTF
DIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTGEMQIENGEVTLGR
SNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTFVHALTGFQNGTLNID
AGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGDIVVDDGAVLSLEGDAAD
LTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNL
HIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFT
DKQQESVMEITSDVDTRSDAAGHGRDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEG
STLTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDI
QSIDAISSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLET
DSLTYLSNVTVNGNLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQ
LVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAY
DYTLVEDNNDWYLRSQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGG
YLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLF
SGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAW
LDSWLQYAWFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQ
DDFTAANRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTIS
DDAVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW