Protein Info for b2182 in Escherichia coli BW25113

Name: bcr
Annotation: bicyclomycin/multidrug efflux system (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 47 to 65 (19 residues), see Phobius details amino acids 77 to 96 (20 residues), see Phobius details amino acids 102 to 123 (22 residues), see Phobius details amino acids 135 to 159 (25 residues), see Phobius details amino acids 165 to 185 (21 residues), see Phobius details amino acids 216 to 240 (25 residues), see Phobius details amino acids 252 to 273 (22 residues), see Phobius details amino acids 285 to 307 (23 residues), see Phobius details amino acids 312 to 332 (21 residues), see Phobius details amino acids 344 to 366 (23 residues), see Phobius details amino acids 372 to 390 (19 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 6 to 390 (385 residues), 540.4 bits, see alignment E=3.3e-166 PF07690: MFS_1" amino acids 16 to 359 (344 residues), 172.5 bits, see alignment E=1.9e-54 PF06609: TRI12" amino acids 30 to 183 (154 residues), 26.5 bits, see alignment E=3.3e-10 PF00083: Sugar_tr" amino acids 47 to 184 (138 residues), 44.8 bits, see alignment E=1.3e-15 TIGR00880: multidrug resistance protein" amino acids 52 to 188 (137 residues), 142.6 bits, see alignment E=8.7e-46

Best Hits

Swiss-Prot: 100% identical to BCR_ECOLI: Bicyclomycin resistance protein (bcr) from Escherichia coli (strain K12)

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to eco:b2182)

MetaCyc: 100% identical to multidrug efflux pump Bcr (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-340; TRANS-RXN-44

Predicted SEED Role

"MFS family multidrug transport protein, bicyclomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P28246 at UniProt or InterPro

Protein Sequence (396 amino acids)

>b2182 bicyclomycin/multidrug efflux system (NCBI) (Escherichia coli BW25113)
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFAL
GQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVIN
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAM
IFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISA
LLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
LSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR