Protein Info for b2154 in Escherichia coli BW25113

Name: yeiG
Annotation: predicted esterase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 2 to 275 (274 residues), 392 bits, E=8.5e-122
• PF00756: Esterase
  • amino acids 21 to 271 (251 residues), 250 bits, E=3e-78
• PF00326: Peptidase_S9
  • amino acids 127 to 275 (149 residues), 29.2 bits, E=6.5e-11

Best Hits

Swiss-Prot: 100% identical to SFGH2_ECOHS: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 100% identity to eco:b2154)

MetaCyc: 100% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.12, 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Sequence Analysis Tools

See P33018 at UniProt or InterPro

Protein Sequence (278 amino acids)

>b2154 predicted esterase (NCBI) (Escherichia coli BW25113)
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFT
TKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDY
LRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPGKYTSVSAFAPIVNPCSVPWG
IKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK