Protein Info for b2150 in Escherichia coli BW25113

Name: mglB
Annotation: methyl-galactoside transporter subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF13407: Peripla_BP_4" amino acids 28 to 299 (272 residues), 220.9 bits, see alignment E=2.2e-69 PF00532: Peripla_BP_1" amino acids 29 to 254 (226 residues), 37.2 bits, see alignment E=2.4e-13

Best Hits

Swiss-Prot: 100% identical to DGAL_SHIFL: D-galactose-binding periplasmic protein (mglB) from Shigella flexneri

KEGG orthology group: K10540, methyl-galactoside transport system substrate-binding protein (inferred from 100% identity to eco:b2150)

MetaCyc: 100% identical to D-galactose/methyl-galactoside ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-18-RXN; TRANS-RXN0-541

Predicted SEED Role

"Galactose/methyl galactoside ABC transport system, D-galactose-binding periplasmic protein MglB (TC 3.A.1.2.3)" in subsystem Bacterial Chemotaxis or Lactose and Galactose Uptake and Utilization (TC 3.A.1.2.3)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AEE5 at UniProt or InterPro

Protein Sequence (332 amino acids)

>b2150 methyl-galactoside transporter subunit (NCBI) (Escherichia coli BW25113)
MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLL
MNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRK
ALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGHPDAEA
RTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAWLSGPNANKIEVVIANNDAMAMG
AVEALKAHNKSSIPVFGVDALPEALALVKSGALAGTVLNDANNQAKATFDLAKNLADGKG
AADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK