Protein Info for b2104 in Escherichia coli BW25113

Name: thiM
Annotation: hydroxyethylthiazole kinase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to THIM_ECOLI: Hydroxyethylthiazole kinase (thiM) from Escherichia coli (strain K12)

KEGG orthology group: K00878, hydroxyethylthiazole kinase [EC: 2.7.1.50] (inferred from 100% identity to eco:b2104)

MetaCyc: 100% identical to hydroxyethylthiazole kinase (Escherichia coli K-12 substr. MG1655)
Hydroxyethylthiazole kinase. [EC: 2.7.1.50]; 2.7.1.50 [EC: 2.7.1.50]; Choline kinase. [EC: 2.7.1.50, 2.7.1.32]

Predicted SEED Role

"Hydroxyethylthiazole kinase (EC 2.7.1.50)" in subsystem Thiamin biosynthesis (EC 2.7.1.50)

MetaCyc Pathways

thiamine diphosphate salvage II (5/5 steps found)
prenylated FMNH₂ biosynthesis (3/3 steps found)
thiamine diphosphate salvage V (3/3 steps found)
thiamine diphosphate salvage IV (yeast) (4/7 steps found)
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
plasmalogen biosynthesis I (aerobic) (1/16 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.32 or 2.7.1.50

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P76423 at UniProt or InterPro

Protein Sequence (262 amino acids)

>b2104 hydroxyethylthiazole kinase (NCBI) (Escherichia coli BW25113)
MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
AAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVAVGALDYRRHFCHELLSFK
PAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEMDYVTDG
HRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLTQEVQA