Protein Info for b2042 in Escherichia coli BW25113
Name: galF
Annotation: predicted subunit with GalU (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GALF_ECOLI: UTP--glucose-1-phosphate uridylyltransferase (galF) from Escherichia coli (strain K12)
KEGG orthology group: K00963, UTP--glucose-1-phosphate uridylyltransferase [EC: 2.7.7.9] (inferred from 100% identity to eco:b2042)MetaCyc: 58% identical to UTP--glucose-1-phosphate uridylyltransferase (Escherichia coli K-12 substr. MG1655)
UTP-monosaccharide-1-phosphate uridylyltransferase. [EC: 2.7.7.64, 2.7.7.9]
Predicted SEED Role
"UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9)" (EC 2.7.7.9)
MetaCyc Pathways
- superpathway of anaerobic sucrose degradation (17/19 steps found)
- colanic acid building blocks biosynthesis (11/11 steps found)
- stachyose degradation (7/7 steps found)
- D-galactose detoxification (3/3 steps found)
- superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
- UDP-α-D-glucose biosynthesis (2/2 steps found)
- sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
- sucrose degradation II (sucrose synthase) (4/5 steps found)
- sucrose biosynthesis II (6/8 steps found)
- type I lipoteichoic acid biosynthesis (S. aureus) (5/17 steps found)
KEGG Metabolic Maps
- Galactose metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.9
Use Curated BLAST to search for 2.7.7.64 or 2.7.7.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P0AAB6 at UniProt or InterPro
Protein Sequence (297 amino acids)
>b2042 predicted subunit with GalU (NCBI) (Escherichia coli BW25113) MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASK NAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPA IGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTK EPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE