Protein Info for b2036 in Escherichia coli BW25113

Name: glf
Annotation: UDP-galactopyranose mutase, FAD/NAD(P)-binding (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR00031: UDP-galactopyranose mutase" amino acids 1 to 365 (365 residues), 568 bits, see alignment E=4.5e-175 PF00070: Pyr_redox" amino acids 5 to 45 (41 residues), 23.7 bits, see alignment 1.1e-08 PF13450: NAD_binding_8" amino acids 6 to 72 (67 residues), 77.8 bits, see alignment E=1.2e-25 PF01593: Amino_oxidase" amino acids 15 to 108 (94 residues), 31.4 bits, see alignment E=2.5e-11 PF03275: GLF" amino acids 147 to 346 (200 residues), 288.3 bits, see alignment E=6.8e-90

Best Hits

Swiss-Prot: 100% identical to GLF_ECOLI: UDP-galactopyranose mutase (glf) from Escherichia coli (strain K12)

KEGG orthology group: K01854, UDP-galactopyranose mutase [EC: 5.4.99.9] (inferred from 100% identity to eco:b2036)

MetaCyc: 100% identical to UDP-galactopyranose mutase (Escherichia coli K-12 substr. MG1655)
UDP-galactopyranose mutase. [EC: 5.4.99.9]

Predicted SEED Role

"UDP-galactopyranose mutase (EC 5.4.99.9)" in subsystem LOS core oligosaccharide biosynthesis or linker unit-arabinogalactan synthesis (EC 5.4.99.9)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37747 at UniProt or InterPro

Protein Sequence (367 amino acids)

>b2036 UDP-galactopyranose mutase, FAD/NAD(P)-binding (NCBI) (Escherichia coli BW25113)
MYDYIIVGSGLFGAVCANELKKLNKKVLVIEKRNHIGGNAYTEDCEGIQIHKYGAHIFHT
NDKYIWDYVNDLVEFNRFTNSPLAIYKDKLFNLPFNMNTFHQMWGVKDPQEAQNIINAQK
KKYGDKVPENLEEQAISLVGEDLYQALIKGYTEKQWGRSAKELPAFIIKRIPVRFTFDNN
YFSDRYQGIPVGGYTKLIEKMLEGVDVKLGIDFLKDKDSLASKAHRIIYTGPIDQYFDYR
FGALEYRSLKFETERHEFPNFQGNAVINFTDANVPYTRIIEHKHFDYVETKHTVVTKEYP
LEWKVGDEPYYPVNDNKNMELFKKYRELASREDKVIFGGRLAEYKYYDMHQVISAALYQV
KNIMSTD