Protein Info for b2017 in Escherichia coli BW25113

Name: yefM
Annotation: orf, hypothetical protein (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 83 PF02604: PhdYeFM_antitox" amino acids 1 to 74 (74 residues), 96 bits, see alignment E=4.8e-32 TIGR01552: prevent-host-death family protein" amino acids 3 to 56 (54 residues), 47.9 bits, see alignment E=3.1e-17

Best Hits

Swiss-Prot: 100% identical to YEFM_ECOLI: Antitoxin YefM (yefM) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b2017)

MetaCyc: 100% identical to YefM antitoxin of the YoeB-YefM toxin-antitoxin pair and DNA binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"YefM protein (antitoxin to YoeB)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P69346 at UniProt or InterPro

Protein Sequence (83 amino acids)

>b2017 orf, hypothetical protein (VIMSS) (Escherichia coli BW25113)
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPAN
ARRLMDSIDSLKSGKGTEKDIIE