Protein Info for b1993 in Escherichia coli BW25113

Name: cobU
Annotation: adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02283: CobU
  • amino acids 3 to 180 (178 residues), 217.4 bits, E=4.8e-69

Best Hits

Swiss-Prot: 100% identical to COBU_ECOLI: Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU) from Escherichia coli (strain K12)

KEGG orthology group: K02231, adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase [EC: 2.7.1.156 2.7.7.62] (inferred from 100% identity to eco:b1993)

MetaCyc: 82% identical to adenosylcobinamide-phosphate guanylyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Adenosylcobinamide-phosphate guanylyltransferase. [EC: 2.7.7.62]; Adenosylcobinamide kinase. [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
• adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (7/9 steps found)
• adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (4/6 steps found)
• adenosylcobinamide-GDP salvage from cobinamide II (4/6 steps found)
• adenosylcobinamide-GDP salvage from assorted adenosylcobamides (1/2 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (16/36 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.156

Use Curated BLAST to search for 2.7.1.156 or 2.7.7.62

Sequence Analysis Tools

See P0AE76 at UniProt or InterPro

Protein Sequence (181 amino acids)

>b1993 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI) (Escherichia coli BW25113)
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVER
WQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLI
AACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKI
K