Protein Info for b1817 in Escherichia coli BW25113

Name: manX
Annotation: fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00824: PTS system, mannose/fructose/sorbose family, IIA component
  • amino acids 2 to 117 (116 residues), 173.3 bits, E=1.7e-55
• PF03610: EIIA-man
  • amino acids 5 to 117 (113 residues), 122.6 bits, E=9.7e-40
• PF03830: PTSIIB_sorb
  • amino acids 164 to 310 (147 residues), 188.9 bits, E=6.5e-60
• TIGR00854: PTS system, mannose/fructose/sorbose family, IIB component
  • amino acids 164 to 311 (148 residues), 189.1 bits, E=4.7e-60

Best Hits

Swiss-Prot: 100% identical to PTNAB_ECOL6: PTS system mannose-specific EIIAB component (manX) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02793, PTS system, mannose-specific IIA component [EC: 2.7.1.69] K02794, PTS system, mannose-specific IIB component [EC: 2.7.1.69] (inferred from 100% identity to eco:b1817)

MetaCyc: 100% identical to mannose-specific PTS enzyme IIAB component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-157 [EC: 2.7.1.199]; 2.7.1.199 [EC: 2.7.1.199]; TRANS-RXN-167 [EC: 2.7.1.199, 2.7.1.193]; TRANS-RXN-165 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]; 2.7.1.191 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
• glycolysis I (from glucose 6-phosphate) (13/13 steps found)
• D-mannose degradation I (2/2 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.191 or 2.7.1.193 or 2.7.1.199 or 2.7.1.69

Sequence Analysis Tools

See P69797 at UniProt or InterPro

Protein Sequence (323 amino acids)

>b1817 fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI) (Escherichia coli BW25113)
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTK
GVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVE
TGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPNDYMVIGLARIDDRLIHGQVAT
RWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERV
MLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIE
LEVRKVSTDPKLKMMDLISKIDK