Protein Info for b1803 in Escherichia coli BW25113

Name: yeaX
Annotation: predicted oxidoreductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF00970: FAD_binding_6" amino acids 9 to 103 (95 residues), 24.2 bits, see alignment E=6.8e-09 PF00175: NAD_binding_1" amino acids 117 to 207 (91 residues), 32.1 bits, see alignment E=3.2e-11 PF00111: Fer2" amino acids 240 to 313 (74 residues), 52.9 bits, see alignment E=5.7e-18

Best Hits

Swiss-Prot: 100% identical to CNTB_ECOLI: Carnitine monooxygenase reductase subunit (yeaX) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b1803)

MetaCyc: 100% identical to carnitine monooxygenase subunit YeaX (Escherichia coli K-12 substr. MG1655)
RXN-18258 [EC: 1.14.13.239]; 1.14.13.239 [EC: 1.14.13.239]; 1.14.13.239 [EC: 1.14.13.239]

Predicted SEED Role

"Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-)" (EC 1.14.13.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.-

Use Curated BLAST to search for 1.14.13.- or 1.14.13.239

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P76254 at UniProt or InterPro

Protein Sequence (321 amino acids)

>b1803 predicted oxidoreductase (NCBI) (Escherichia coli BW25113)
MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVIVQMSDGDNQYSNAYSL
LSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTPNNLFALIPSARKHLFIAG
GIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELVQHPQAEKVHLHHSSTGTRLE
LARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAADTLHFEQFAIEDKTGDAFTLVLA
RSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCGTCETAILEGEADHRDQYFSDEERA
SQQSMLICCSRAKGKRLVLDL