Protein Info for b1800 in Escherichia coli BW25113
Name: yeaU
Annotation: predicted dehydrogenase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to DMLA_ECOLI: D-malate dehydrogenase [decarboxylating] (dmlA) from Escherichia coli (strain K12)
KEGG orthology group: K07246, tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase [EC: 1.1.1.83 1.1.1.93 4.1.1.73] (inferred from 100% identity to eco:b1800)MetaCyc: 100% identical to D-malate/3-isopropylmalate dehydrogenase (decarboxylating) (Escherichia coli K-12 substr. MG1655)
Tartrate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73]; 3-isopropylmalate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85]; D-malate dehydrogenase (decarboxylating). [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85, 1.1.1.83]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- D-malate degradation (1/1 steps found)
- gentisate degradation II (1/4 steps found)
- methylgallate degradation (2/6 steps found)
- protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
- butanol and isobutanol biosynthesis (engineered) (2/8 steps found)
- superpathway of vanillin and vanillate degradation (3/10 steps found)
- syringate degradation (3/12 steps found)
- aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Butanoate metabolism
- Glyoxylate and dicarboxylate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.85
Use Curated BLAST to search for 1.1.1.83 or 1.1.1.85 or 1.1.1.93 or 4.1.1.73
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P76251 at UniProt or InterPro
Protein Sequence (361 amino acids)
>b1800 predicted dehydrogenase (NCBI) (Escherichia coli BW25113) MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIIL R