Protein Info for b1617 in Escherichia coli BW25113

Name: uidA
Annotation: beta-D-glucuronidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 603 PF02837: Glyco_hydro_2_N" amino acids 12 to 180 (169 residues), 218.5 bits, see alignment E=8.2e-69 PF00703: Glyco_hydro_2" amino acids 184 to 272 (89 residues), 34.9 bits, see alignment E=3.5e-12 PF02836: Glyco_hydro_2_C" amino acids 274 to 593 (320 residues), 379.7 bits, see alignment E=1.4e-117

Best Hits

Swiss-Prot: 100% identical to BGLR_ECOLI: Beta-glucuronidase (uidA) from Escherichia coli (strain K12)

KEGG orthology group: K01195, beta-glucuronidase [EC: 3.2.1.31] (inferred from 100% identity to eco:b1617)

MetaCyc: 100% identical to beta-glucuronidase (Escherichia coli K-12 substr. MG1655)
Beta-glucuronidase. [EC: 3.2.1.31]

Predicted SEED Role

"Beta-glucuronidase (EC 3.2.1.31)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.2.1.31)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P05804 at UniProt or InterPro

Protein Sequence (603 amino acids)

>b1617 beta-D-glucuronidase (NCBI) (Escherichia coli BW25113)
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADI
RNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
NTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
GKQ