Protein Info for b1590 in Escherichia coli BW25113

Name: ynfH
Annotation: oxidoreductase, membrane subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 46 to 67 (22 residues), see Phobius details amino acids 87 to 106 (20 residues), see Phobius details amino acids 116 to 138 (23 residues), see Phobius details amino acids 150 to 169 (20 residues), see Phobius details amino acids 181 to 204 (24 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 250 to 272 (23 residues), see Phobius details PF04976: DmsC" amino acids 1 to 274 (274 residues), 426.8 bits, see alignment E=1.9e-132

Best Hits

Swiss-Prot: 100% identical to YNFH_ECOLI: Anaerobic dimethyl sulfoxide reductase chain YnfH (ynfH) from Escherichia coli (strain K12)

KEGG orthology group: K07312, Tat-targeted selenate reductase subunit YnfH (inferred from 100% identity to eco:b1590)

MetaCyc: 100% identical to putative menaquinol dehydrogenase (Escherichia coli K-12 substr. MG1655)
Selenate reductase. [EC: 1.97.1.9]

Predicted SEED Role

"Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3)" (EC 1.8.5.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.5.3, 1.97.1.9

Use Curated BLAST to search for 1.8.5.3 or 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P76173 at UniProt or InterPro

Protein Sequence (284 amino acids)

>b1590 oxidoreductase, membrane subunit (NCBI) (Escherichia coli BW25113)
MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIVRGMFFLWLLMGVGFIA
SVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWLVAVIGKMPQALGKLWLLF
SMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLSGPILAAAILRAARVTFNTTP
FAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVPDYASLQVWRVVLLCAGLGCWLC
PLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHMTVGMAIAG