Protein Info for b1588 in Escherichia coli BW25113

Name: ynfF
Annotation: oxidoreductase subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 807 signal peptide" amino acids 1 to 46 (46 residues), see Phobius details TIGR02166: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family" amino acids 14 to 807 (794 residues), 1286.2 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 52 to 111 (60 residues), 47.3 bits, see alignment 2.4e-16 PF00384: Molybdopterin" amino acids 114 to 565 (452 residues), 217.9 bits, see alignment E=3.3e-68 PF01568: Molydop_binding" amino acids 686 to 799 (114 residues), 92.9 bits, see alignment E=2e-30

Best Hits

Swiss-Prot: 100% identical to YNFF_ECOLI: Probable dimethyl sulfoxide reductase chain YnfF (ynfF) from Escherichia coli (strain K12)

KEGG orthology group: K07310, Tat-targeted selenate reductase subunit YnfF [EC: 1.97.1.9] (inferred from 100% identity to ebd:ECBD_2058)

MetaCyc: 65% identical to dimethyl sulfoxide reductase subunit A (Escherichia coli K-12 substr. MG1655)
DIMESULFREDUCT-RXN [EC: 1.8.5.3]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.5.3, 1.97.1.9

Use Curated BLAST to search for 1.8.5.3 or 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77783 at UniProt or InterPro

Protein Sequence (807 amino acids)

>b1588 oxidoreductase subunit (RefSeq) (Escherichia coli BW25113)
MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEAPVEEKAVWSSCT
VNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRGRSIRRRMNHPDRLKYPMK
RVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGGNITNSNVPYRL
MNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGG
VTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLITENMVD
QPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEGPDGIAKTPEWAAKITSIPAEKIIQLA
REIGSAKPAYICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGVREGSWDLGVEW
FPMLENPVKTQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHG
DINHTHEVLQDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVI
LAQPATSAKFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPD
YEEMKTTGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKD
EIIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPI
DAQARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSI
NILTSHRPSPLAKGNPSHSNLVQIEKV