Protein Info for b1539 in Escherichia coli BW25113

Name: ydfG
Annotation: L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 187 (186 residues), 181.6 bits, E=2.5e-57
• PF08659: KR
  • amino acids 3 to 170 (168 residues), 48.4 bits, E=2.1e-16
• PF01370: Epimerase
  • amino acids 3 to 134 (132 residues), 27.9 bits, E=3.1e-10
• PF13561: adh_short_C2
  • amino acids 7 to 218 (212 residues), 122.6 bits, E=4.2e-39

Best Hits

Swiss-Prot: 100% identical to YDFG_ECOLI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Escherichia coli (strain K12)

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 100% identity to eco:b1539)

MetaCyc: 100% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (16/18 steps found)
• uracil degradation III (5/5 steps found)
• L-threonine degradation III (to methylglyoxal) (3/3 steps found)
• aminopropanol phosphate biosynthesis II (3/4 steps found)
• β-alanine degradation III (1/2 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Sequence Analysis Tools

See P39831 at UniProt or InterPro

Protein Sequence (248 amino acids)

>b1539 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) (Escherichia coli BW25113)
MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAA
IEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPG
MVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAVRVTDIEPGLV
GGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY
AGLNVHRQ