Protein Info for b1494 in Escherichia coli BW25113

Name: pqqL
Annotation: predicted peptidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 931 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00675: Peptidase_M16" amino acids 47 to 202 (156 residues), 136 bits, see alignment E=1e-43 PF05193: Peptidase_M16_C" amino acids 207 to 386 (180 residues), 102.1 bits, see alignment E=3.5e-33 amino acids 689 to 862 (174 residues), 78.6 bits, see alignment E=5.6e-26

Best Hits

Swiss-Prot: 100% identical to PQQL_ECOLI: Probable zinc protease PqqL (pqqL) from Escherichia coli (strain K12)

KEGG orthology group: K07263, zinc protease [EC: 3.4.24.-] (inferred from 100% identity to eco:b1494)

Predicted SEED Role

"Probable zinc protease pqqL (EC 3.4.99.-)" (EC 3.4.99.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-, 3.4.99.-

Use Curated BLAST to search for 3.4.24.- or 3.4.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P31828 at UniProt or InterPro

Protein Sequence (931 amino acids)

>b1494 predicted peptidase (NCBI) (Escherichia coli BW25113)
MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNL
WLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYD
ETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAKWRTSQ
ARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALA
LIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSF
IEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDA
ANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTP
FLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNEVAAKKALSPAAILAL
EKEYANKKLAAYVFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGE
EQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKV
SGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFA
QQMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALI
TRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLPTRMA
LDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLAN
EVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQL
LKQMTVENVNTAVKQYLSHPVNTYTGVLLPK