Protein Info for b1493 in Escherichia coli BW25113

Name: gadB
Annotation: glutamate decarboxylase B, PLP-dependent (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 TIGR01788: glutamate decarboxylase" amino acids 16 to 448 (433 residues), 692.5 bits, see alignment E=9.6e-213 PF00282: Pyridoxal_deC" amino acids 33 to 383 (351 residues), 372.2 bits, see alignment E=1.2e-115

Best Hits

Swiss-Prot: 100% identical to DCEB_ECOLI: Glutamate decarboxylase beta (gadB) from Escherichia coli (strain K12)

KEGG orthology group: K01580, glutamate decarboxylase [EC: 4.1.1.15] (inferred from 100% identity to eco:b1493)

MetaCyc: 100% identical to glutamate decarboxylase B (Escherichia coli K-12 substr. MG1655)
Glutamate decarboxylase. [EC: 4.1.1.15]

Predicted SEED Role

"Glutamate decarboxylase (EC 4.1.1.15)" in subsystem Acid resistance mechanisms (EC 4.1.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.15

Use Curated BLAST to search for 4.1.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P69910 at UniProt or InterPro

Protein Sequence (466 amino acids)

>b1493 glutamate decarboxylase B, PLP-dependent (NCBI) (Escherichia coli BW25113)
MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNL
ATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVG
TNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREI
PMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDM
HIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKL
GPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVV
MRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT