Protein Info for b1415 in Escherichia coli BW25113

Name: aldA
Updated annotation (from data): Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21)
Rationale: Specific phenotype: utilization of L-Fucose. Also detoxifies glycolaldehyde (PMC4585216)
Original annotation: aldehyde dehydrogenase A, NAD-linked (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 22 to 475 (454 residues), 557 bits, E=1.4e-171

Best Hits

Swiss-Prot: 100% identical to ALDA_ECOLI: Lactaldehyde dehydrogenase (aldA) from Escherichia coli (strain K12)

KEGG orthology group: K07248, lactaldehyde dehydrogenase / glycolaldehyde dehydrogenase [EC: 1.2.1.21 1.2.1.22] (inferred from 100% identity to eco:b1415)

MetaCyc: 100% identical to aldehyde dehydrogenase A (Escherichia coli K-12 substr. MG1655)
Lactaldehyde dehydrogenase. [EC: 1.2.1.22]; Glycolaldehyde dehydrogenase. [EC: 1.2.1.22, 1.2.1.21]

Predicted SEED Role

"Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21)" in subsystem L-rhamnose utilization or Methylglyoxal Metabolism (EC 1.2.1.21, EC 1.2.1.22)

MetaCyc Pathways

• superpathway of fucose and rhamnose degradation (12/12 steps found)
• superpathway of glycol metabolism and degradation (7/7 steps found)
• D-arabinose degradation II (4/4 steps found)
• L-lactaldehyde degradation (aerobic) (2/2 steps found)
• ethylene glycol degradation (2/2 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• methylglyoxal degradation IV (2/3 steps found)
• methylglyoxal degradation V (2/3 steps found)
• lactate biosynthesis (archaea) (3/5 steps found)
• L-rhamnose degradation II (4/8 steps found)
• superpathway of pentose and pentitol degradation (18/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.21 or 1.2.1.22

Sequence Analysis Tools

See P25553 at UniProt or InterPro

Protein Sequence (479 amino acids)

>b1415 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (Escherichia coli BW25113)
MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEW
EALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWAR
RYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEF
TPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMAT
AAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQ
FVNRLGEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEG
KGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNL
NVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQS