Protein Info for b1404 in Escherichia coli BW25113

Name: insI-2
Annotation: IS30 transposase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 PF13936: HTH_38" amino acids 58 to 98 (41 residues), 66.9 bits, see alignment 1.6e-22 PF00665: rve" amino acids 229 to 320 (92 residues), 53.4 bits, see alignment E=4e-18

Best Hits

Swiss-Prot: 100% identical to INSI4_ECOLI: Transposase InsI for insertion sequence element IS30D (insI4) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b4284)

MetaCyc: 100% identical to IS30 transposase (Escherichia coli K-12 substr. MG1655)
2.7.7.-

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0CF89 at UniProt or InterPro

Protein Sequence (383 amino acids)

>b1404 IS30 transposase (NCBI) (Escherichia coli BW25113)
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELAR
HLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNN
RPRKTLKFKTPKEIIERGVALTD